antibodies against integrin αv Search Results


rat monoclonal antibody against mouse αv integrin subunit (rm47  (Becton Dickinson)
90
Becton Dickinson rat monoclonal antibody against mouse αv integrin subunit (rm47
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Rat Monoclonal Antibody Against Mouse αv Integrin Subunit (Rm47, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat monoclonal antibody against mouse αv integrin subunit (rm47/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
rat monoclonal antibody against mouse αv integrin subunit (rm47 - by Bioz Stars, 2026-03
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mouse antibodies against human integrins α1, αv β4  (Becton Dickinson)
90
Becton Dickinson mouse antibodies against human integrins α1, αv β4
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Mouse Antibodies Against Human Integrins α1, αv β4, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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monoclonal antibody (mab) 69-6-5 against the αv integrin subunit  (Immunotec inc)
90
Immunotec inc monoclonal antibody (mab) 69-6-5 against the αv integrin subunit
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Monoclonal Antibody (Mab) 69 6 5 Against The αv Integrin Subunit, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal antibody (mab) 69-6-5 against the αv integrin subunit/product/Immunotec inc
Average 90 stars, based on 1 article reviews
monoclonal antibody (mab) 69-6-5 against the αv integrin subunit - by Bioz Stars, 2026-03
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antibodies against β1, β3, α1, α2, α5, α6, αv integrin  (Becton Dickinson)
90
Becton Dickinson antibodies against β1, β3, α1, α2, α5, α6, αv integrin
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Antibodies Against β1, β3, α1, α2, α5, α6, αv Integrin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
antibodies against β1, β3, α1, α2, α5, α6, αv integrin - by Bioz Stars, 2026-03
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monoclonal antibodies against the αv and β3 integrin subunits  (PASCO)
90
PASCO monoclonal antibodies against the αv and β3 integrin subunits
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Monoclonal Antibodies Against The αv And β3 Integrin Subunits, supplied by PASCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal antibodies against the αv and β3 integrin subunits/product/PASCO
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primary antibodies against αv integrin  (Covance)
90
Covance primary antibodies against αv integrin
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Primary Antibodies Against αv Integrin, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against integrin subunits αv 551187  (Becton Dickinson)
90
Becton Dickinson antibodies against integrin subunits αv 551187
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Antibodies Against Integrin Subunits αv 551187, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phycoerythrin (pe)-labelled antibodies against integrin subunits αv  (Becton Dickinson)
90
Becton Dickinson phycoerythrin (pe)-labelled antibodies against integrin subunits αv
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Phycoerythrin (Pe) Labelled Antibodies Against Integrin Subunits αv, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibodies against the αv integrin subunit  (Telios Pharmaceuticals)
90
Telios Pharmaceuticals monoclonal antibodies against the αv integrin subunit
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
Monoclonal Antibodies Against The αv Integrin Subunit, supplied by Telios Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal antibodies against the αv integrin subunit/product/Telios Pharmaceuticals
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first antibodies against integrin α1, α2, α5, αv, β1, and β3  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc first antibodies against integrin α1, α2, α5, αv, β1, and β3
Localization of CD9 and the <t>αv</t> subunit of αvβ3 <t>integrin</t> on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.
First Antibodies Against Integrin α1, α2, α5, αv, β1, And β3, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Localization of CD9 and the αv subunit of αvβ3 integrin on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.

Journal: The Journal of Biological Chemistry

Article Title: Oviductosome-Sperm Membrane Interaction in Cargo Delivery

doi: 10.1074/jbc.M114.633156

Figure Lengend Snippet: Localization of CD9 and the αv subunit of αvβ3 integrin on sperm and OVS. A, distribution of CD9 and αv integrin on sperm studied by immunofluorescence and conventional confocal microscopy. CD9 (green, a-b) and αv integrin (red, c-d) localized on the midpiece, the proximal principal piece, and the over the acrosome. Sperm nuclei were stained with DAPI (blue) and are seen in the merged images in b and d. IgG control did not give any signal (e-f).). Representative images from a total of n = ∼10 sperm in each group. B, Western blotting analysis performed with anti-CD9 (a) and anti-αv integrin (b) antibodies on OVS recovered from oviductal fluid, proestrus, and estrus combined and metestrus and diestrus combined, as well as hormonally induced estrus (I.E OVS), using epididymosomes (EPI) and uterus as positive controls. The 24-kDa CD9 and the 130-kDa αv integrin are present in OVS from all stages. C, co-localization of FM4–64FX-labeled OVS and αv integrin on caudal sperm following co-incubation and in vitro fusion, using three-dimensional SR-SIM. Immunofluorescence reveals that transferred αv integrin potentially co-localizes with OVS on the sperm membrane. The localization pattern of αv is similar to PMCA4a, over the acrosome, neck (blue arrow), mid-piece, and the proximal principal piece of the sperm flagellum. The yellow arrows show potential overlap between the red FM4–64FX and the green αv integrin signal giving yellow on the head (a) and on the midpiece (b), since the presence of endogenous αv integrin cannot be ruled out at these sites. The green signal on the head (b) and principal piece (a, b) is unequivocally endogenous αv integrin. Sperm nuclei were stained blue with DRAQ5. Images were captured with a 63× Plan-Apochromatic oil immersion objective (numerical aperture of 1.4). Representative images from a total of n = ∼15 sperm Scale bars, 5 μm.

Article Snippet: To perform a function-blocking assay, a rat monoclonal antibody against mouse αv integrin subunit (RM47) and its isotype IgG 1κ (R3–34) were obtained from BD Bioscience (San Diego, CA).

Techniques: Immunofluorescence, Confocal Microscopy, Staining, Western Blot, Labeling, Incubation, In Vitro